ISO 15593:2001 pdf download
ISO 15593:2001 pdf download.Environmental tobacco smoke—
Estimation of its contribution to respirable suspended particles- Determination of particulate matter by ultraviolet absorbance and by fluorescence.
6.6.1 Primary standard of THBP (1 000 ig/ml), prepared by weighing 100 mg of THBP (6.2) directly into a
100 ml volumetric flask, diluting to the mark with methanol, and shaking to mix.
6.6.2 Secondary standard of THBP (16 pg/mI), prepared by transferring 4,00 ml of the primary standard (6.6.1) to a 250 ml volumetric flask, diluting to the mark with methanol, and shaking to mix.
6.6.3 Working standards of THBP
Prepare five working standards covering the expected concentration range of the samples by transferring defined volumes of the secondary standard (6.6.2) to 100 ml volumetric flasks, diluting to the mark with methanol, and shaking to mix.
Typical volumes used are 1 ml, 2 ml, 5 ml, 10 ml, 20 ml and 40 ml that yield UVPM standards of THBP content of 0,16 pg/mI, 0,32 pg/mI, 0,80 pg/mI, 1,60 pg/mI, 3,20 pg/mI and 6,40 pg/mI, respectively. Of these, select either the five lowest or the five highest in concentration to cover the expected range of samples.
6.7 FPM surrogate standard solutions
Store all standard solutions in low-actinic borosilicate glass screw-cap jars in a refrigerator (at about 4 °C) when not in use. Prepare fresh standards from scopoletin at least every 6 months.
6.7.1 Primary standard of scopoletin (350 pg/mI), prepared by weighing 35 mg of scopoletin [assuming 100 % purity scopoletin (6.3)] directly into a 100 ml volumetric flask, diluting to the mark with methanol, and shaking to mix.
6.7.2 Secondary standard of scopoletin (3,50 pg/mI), prepared by transferring 1,00 ml of the primary standard (6.7.1) to a 100 ml volumetric flask, diluting to the mark with methanol, and shaking to mix. This secondary standard is also the highest level working standard.
6.7.3 Tertiary standard of scopoletin (0,350 pg/mI), prepared by transferring 10,00 ml of the secondary standard (6.7.2) to a 100 ml volumetric flask, diluting to the mark with methanol, and shaking to mix. This tertiary standard is also one of the working standards.
6.7.4 Working standards of scopoletin
Prepare five working standards covering the expected concentration range of the samples by transferring defined volumes of the secondary standard (6.7.2) and the tertiary standard (6.7.3) to 100 ml volumetric flasks, diluting to the mark with methanol, and shaking to mix.
Typical volumes used are 1 ml and 3 ml of the tertiary standard and 1 ml, 3 ml and 30 ml of the secondary standard, that yield FPM standards of scopoletin content of 0,003 5 pg/mI, 0,0105 pg/mI, 0,035 pg/mI, 0,105 pg/mI, 0,350 pg/mI (the tertiary standard), 1,05 pg/mI and 3,50 pg/mI (the secondary standard). From this range of working standards, select either the five lowest or the five highest levels to cover the expected concentration range of the samples.
6.8 Glycerol solution
Prepare an aqueous solution of glycerol with a mass fraction of 80,0 % by mixing 800 g of glycerol (6.4) with 200 g distilled, deionized water. Prepare a fresh solution at least every 12 months.
7 Apparatus
Usual laboratory apparatus and, in particular, the following items.
7.1 Sample collection system
7.1.1 Polytetrafluoroethylene (PTFE) membrane filter, of pore size 1,0 pm and diameter 37 mm.
The PTFE membrane is bonded to a high density polyethylene support net, referred to as the filter backing, to improve durability and handling ease.
7.1.2 Filter cassette, of black, opaque, conductive polypropylene in a three-piece configuration consisting of a
12,7 mm spacer ring inserted between the top (inlet) and bottom (outlet) pieces.
The filter cassette holds the PTFE membrane filter during sampling. All connections to the filter cassette are made with flexible (e.g. plastic) tubing.
7.1.3 Barometer and thermometer, for taking pressure and temperature readings at the sampling site.
7.1.4 Bubble flowmeter or mass flowmeter, for calibration of the sampling pump.
7.1.5 Personal sampling pump, constant-flow air sampling pump, calibrated for a flow rate dependent upon the separating characteristics of the impactor or cyclone in use (7.1.6).
7.1.6 Inertial impactor or cyclone, with nominal cut point of 4,0 urn at the specified flow rate.
If the alternative definition of RSP is used (see 3.2), ensure that the impactor or cyclone is compatible with this definition.
7.1.7 Stopcock grease, for coating impactor plates.
7.2 Analytical system
7.2.1 High-performance liquid chromatography (HPLC) system, consisting of a solvent-delivery system, autosampler, ultraviolet detector, fluorescence detector, peak integration system, and 3,0 m stainless-steel tubing with 0,23 mm inside diameter.
No HPLC analytical column is used. If this analysis is attempted using an ultraviolet spectrometer, a cell with path length of at least 40 mm is recommended.
7.2.2 Sample containers, consisting of low-actinic borosilicate glass autosampler vials, of 4 ml capacity, with screw caps and PTFE-Iined septa.
7.3 Microgram balance, for weighing filters, accurate to 1 ig.
7.4 Desiccator cabinet, for use as a humidity-controlled chamber where filters are stored prior to weighing.
7.5 Static inhibitor, for removing static charge from filters.
7.6 Filter forceps, for handling filters.
7.7 Shaking device, with wrist-action for solvent extraction.